ABSTRACT
OBJECTIVE: To develop an HPLC method for simultaneous determination of ginkgolic acids(C13:0, C15:1, C17:2, C15:0, C17:1) in Ginkgo Folium. METHODS: The HPLC analysis was carried out on Phenomenex Luna C18(4.6 mm × 250 mm, 5 μm) column with acetonitrile-0.1% phosphoric acid (90:10) as mobile phase eluted at the flow rate of 1.0 mL · min-1. The detective wavelength was set at 310 nm, and the column temperature was set at 30℃. RESULTS: The calibration curve was linear within the range of 1.47-29.40 μg · mL-1 for C13:0, 6.05 -121.00 μg · mL-1 for C15:1 and 8.00 - 160.00 μg · mL-1 for C17:1, respectively. The average recoveries for the three marker compounds are 98.6% - 100.1%. CONCLUSION: This method is simple, accurate, and practical for the quality control of Ginkgo Folium. There are some differences in the contents of the five marker compounds from different producing areas and different collection periods.